It distresses me to no end that I've recently read papers showing certain freindly bactria wipe out C.Diff. Unfortunately in all cases big phara companies like the one that makes vanc. buys out the guy that discovered it then ties it up for untold years in patents and trials.
Meanwhile if we had a source for these specific probiotics, disregarding patent law, then we could all cure ourselfs and save billions.
Cures for C.Diff
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anlockwood
- Long Time Contributor
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Bry
One example:
Notice from the following link: "These groups are selected because their relative high frequencies as isolates from persons or humans..."
...Strains M3 and M23 (isolated from humans) ...M3 animals remain disease free for at least 100 days after challenge with highly toxigenic strains...very high level of protection and the fact that the protection is extremely durable...
Strains of C. difficile from the M group are suitable for practice of the invention, because of their ability to colonize well. Such strains are also useful for therapeutic purposes to treat patients who have had relapses of C. difficile diarrhea following treatment with antibiotics such as vancomycin or metronidazole. Such patients are exceedingly difficult to cure and are preferred choices for administration of the non-toxigenic strains. Such therapy is preferably administered after first treating the patient with vancomycin or metronidazole to reduce the population of toxigenic C. difficile and to control diarrhea. The vancomycin or metronidazole is stopped for 12-24 hr, and then the non-toxigenic strain of C. difficile is administered orally in the same manner as described for the preventive use of the strain. Repeated doses or higher doses of non-toxigenic C. difficile may be required for treatment as compared to prevention of CDAD. Strains M, T, C, AP, P, S and combinations thereof are suitable M strains of C. difficile are preferred for treatment of relapses because of the known high rate of colonization by M strains in humans, and the low rate of C. difficile disease in humans colonized by non-toxigenic strains of C. difficile.
...
The remaining five hamsters were challenged 60 days after receiving the protective strain M3 with 100 cfu per animal of toxigenic C. difficile strain Bl by oral gavage as described in Section B. Although M3 could no longer be detected in the stool pellets of these animals, they remained well and developed no ill-effects as a result of the Bl challenge. The hamsters were followed for an additional 92-100 days following Bl challenge and developed no evidence of illness. At postmortem examination, there was no evidence of cecitis or colitis. M3 was isolated from the cecum of one animal 159 days after M3 inoculation, which was 117 days after the last positive fecal pellet culture for M3.
Thus, the protection afforded by M3 is extremely durable, as the hamsters were completely protected from challenge with highly virulent toxigenic strains Bl and J9 for 54-60 days after becoming colonized with strain M3...
Note strain M3 was isolated from health humans. I also read elsewhere that M3 was the only Non-Toxigenic Clostridium Difficile that has higher binding properties to the mucous membranes then does the highly toxic strains which also show very high binding abilities.
"None of the probes for toxin A and toxin B genes showed hybridization. Thus, M3 was found to contain neither the gene for toxin A nor the gene for toxin B.
"The phenotypic and genotypic evidence indicates M3 does not produce toxins A and B and does not contain the genes coding for these two toxins. The absence of these virulence factors provides further confirmation that the administration of M3 to human patients is safe.
http://www.google.com/patents/pdf/Metho ... UII6pCMehA
And here we see ViroPharma bought the rights to develop the M3 strain and has tied it up since 2006 with next to no news about it since.
http://www.medicalnewstoday.com/articles/46046.php
You can imagine if M3 strain where available for ingestion, sales of vanc. would drop and others might learn how to grow the strain themselves (from the capsules containing live M3) if their Doctor could prescribe it, hence I suspect it will be a very long time (ten or more years, if at all) before M3 becomes available. Remember M3 is found in the feces of many health humans.
I have read other unrelated examples which I will try to locate and post later.
Notice from the following link: "These groups are selected because their relative high frequencies as isolates from persons or humans..."
...Strains M3 and M23 (isolated from humans) ...M3 animals remain disease free for at least 100 days after challenge with highly toxigenic strains...very high level of protection and the fact that the protection is extremely durable...
Strains of C. difficile from the M group are suitable for practice of the invention, because of their ability to colonize well. Such strains are also useful for therapeutic purposes to treat patients who have had relapses of C. difficile diarrhea following treatment with antibiotics such as vancomycin or metronidazole. Such patients are exceedingly difficult to cure and are preferred choices for administration of the non-toxigenic strains. Such therapy is preferably administered after first treating the patient with vancomycin or metronidazole to reduce the population of toxigenic C. difficile and to control diarrhea. The vancomycin or metronidazole is stopped for 12-24 hr, and then the non-toxigenic strain of C. difficile is administered orally in the same manner as described for the preventive use of the strain. Repeated doses or higher doses of non-toxigenic C. difficile may be required for treatment as compared to prevention of CDAD. Strains M, T, C, AP, P, S and combinations thereof are suitable M strains of C. difficile are preferred for treatment of relapses because of the known high rate of colonization by M strains in humans, and the low rate of C. difficile disease in humans colonized by non-toxigenic strains of C. difficile.
...
The remaining five hamsters were challenged 60 days after receiving the protective strain M3 with 100 cfu per animal of toxigenic C. difficile strain Bl by oral gavage as described in Section B. Although M3 could no longer be detected in the stool pellets of these animals, they remained well and developed no ill-effects as a result of the Bl challenge. The hamsters were followed for an additional 92-100 days following Bl challenge and developed no evidence of illness. At postmortem examination, there was no evidence of cecitis or colitis. M3 was isolated from the cecum of one animal 159 days after M3 inoculation, which was 117 days after the last positive fecal pellet culture for M3.
Thus, the protection afforded by M3 is extremely durable, as the hamsters were completely protected from challenge with highly virulent toxigenic strains Bl and J9 for 54-60 days after becoming colonized with strain M3...
Note strain M3 was isolated from health humans. I also read elsewhere that M3 was the only Non-Toxigenic Clostridium Difficile that has higher binding properties to the mucous membranes then does the highly toxic strains which also show very high binding abilities.
"None of the probes for toxin A and toxin B genes showed hybridization. Thus, M3 was found to contain neither the gene for toxin A nor the gene for toxin B.
"The phenotypic and genotypic evidence indicates M3 does not produce toxins A and B and does not contain the genes coding for these two toxins. The absence of these virulence factors provides further confirmation that the administration of M3 to human patients is safe.
http://www.google.com/patents/pdf/Metho ... UII6pCMehA
And here we see ViroPharma bought the rights to develop the M3 strain and has tied it up since 2006 with next to no news about it since.
http://www.medicalnewstoday.com/articles/46046.php
You can imagine if M3 strain where available for ingestion, sales of vanc. would drop and others might learn how to grow the strain themselves (from the capsules containing live M3) if their Doctor could prescribe it, hence I suspect it will be a very long time (ten or more years, if at all) before M3 becomes available. Remember M3 is found in the feces of many health humans.
I have read other unrelated examples which I will try to locate and post later.
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anlockwood
- Long Time Contributor
- Posts: 456
- Joined: Sat Sep 06, 2008 9:10 am
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